The placement of the HCT gene on female LG9 did not increase the number of bridge mark ers, nor did it affect marker selleckbio order. However, it did succeed in filling a large gap, and in reducing the mean inter marker distance. Increasing marker density by the addition of genes to a map can be accomplished via the exploitation of mapping populations which segregate for traits and markers in common across the populations. We are currently constructing further genetic maps based on combinations between Romanesco C3 and either cultivated or wild cardoon accessions, prima rily as a means of initiating comparative QTL mapping. Within gene markers, such as the ones described here for the HCT and HQT genes, are particularly suitable for gen eral mapping, and should prove useful as anchor points among diverse populations.
Conclusion A novel acyltransferase Inhibitors,Modulators,Libraries involved in the biosynthesis of CGA in globe artichoke has been isolated Inhibitors,Modulators,Libraries and character ized. Its activity and involvement in CGA biosynthesis have been confirmed by heterologous expression assays, demonstrating that it can use either p coumaroyl CoA or caffeoyl CoA as an acyl donor, and quinic acid as an acceptor. We previously observed that the PP metabolism can be induced by UV C irradiation, whose effect on the transcription level of the HCT Inhibitors,Modulators,Libraries and HQT genes has been investigated. The HQT as well as HCT genes have been located in our previously developed globe artichoke genetic maps. the linkage analyses of genes having known biochemical function can help elucidate the complexity of plant secondary metabolism.
This work is a further contribution in the understanding of the genetic basis of phenylpropanoid biosynthesis in C. cardunculus. our future research activity will be focused on the analysis of the expression in vivo of both HQT and HCT, as well as on isolating further acyltrans ferases involved in the phenylpropanoid pathway of the species. Methods Plant material Inhibitors,Modulators,Libraries and RNA extraction Leaves of globe artichoke, and cultivated cardoon were collected from experimental fields in Scalenghe, Torino. Total RNA was extracted from Inhibitors,Modulators,Libraries approximately 100 mg fresh tissue using the Trizol reagent, following the manufacturers instructions. Final RNA con centration was determined by spectrophotometry, and its integrity was assessed by electrophoresis in 1% for maldehyde agarose gel.
Isolation and cloning of full length full article cDNA of globe artichoke and cardoon Reverse transcription from both globe artichoke and car doon total RNA was achieved using poly primer and M MuLV RNaseH RT, following the manufacturers instructions. The PCR amplification of cDNA sequences was performed as described in Comino et al. using primers designed according to the CODEHOP strategy. A first amplification step was performed using as primers CODhqtFor and COD hqtRev, designed on conserved regions after alignment of HQT amino acid sequences available in Genebank Nicotiana tabacum and Lycopersicum esculen tum.