The proposed method was validated as per ICH guidelines.[13�C15]. Figure 1 Structure of Olmesartan Medoxomil Figure 2 Structure of Metoprolol Succinate EXPERIMENTAL Materials OLME and METO were supplied as a gift sample by Cadila Healthcare Ltd., Ahmedabad, selleckchem Cabozantinib India. All chemicals and reagents used were of AR grade and purchased from Merck Chemicals, Mumbai. All the solvents used were of HPLC grade. ACN was purchased from S D Fine-chem Ltd., Mumbai and TFA from Sisco Research Lab Pvt. Ltd., Mumbai. Commercial formulation, Olmax-M tablet manufactured by Glenmark Pharmaceuticals Ltd., Mumbai, containing 20 mg OLME and 25 mg METO was purchased from local market. Instrumentation The HPLC system (Model-Agilent 1200 series) consisted of a Binary pump. The detector consisted of UV/VIS PDA Inhibitors,Modulators,Libraries detector operated at a wavelength of 220 nm.

Data was integrated using Chemstation software. The column Inhibitors,Modulators,Libraries used was YMC-Pack CN (250 �� 4.6 mm, 5.0 ��m) and the injection volume was 20 ��L. Preparation of standard stock solutions The standard stock solutions were prepared individually. Accurately weighed OLME (20 mg) and METO (25 mg) were transferred to separate 100 ml volumetric flasks and then the volume was made up to the mark with ACN: Water (1:1). The stock solutions were further diluted with ACN: Water (1:1) to obtain a solution of OLME (20 ��g/ml) and METO (25 ��g/ml), respectively. METHOD VALIDATION Validation Inhibitors,Modulators,Libraries of the optimized HPLC method was carried out with respect to the following parameters: Linearity and range Linearity of the method was established by injecting six concentrations of the drugs prepared in the ACN: Water (1:1) in the range 5 to 35 ��g/ml for both OLME and METO in six replicates into the HPLC Inhibitors,Modulators,Libraries system keeping the injection volume constant.

The peak areas were plotted against the corresponding concentrations to obtain the calibration graphs. Precision The precision of the method was verified by Inhibitors,Modulators,Libraries repeatability and intermediate GSK-3 precision studies. Repeatability studies were performed by analysis of three different concentrations (15, 20 and 25 ��g/ml for OLME and METO) of the drugs three times on the same day (intra-day) and three consecutive days (inter-day). Limit of detection and limit of quantitation To determine the limits of detection (LOD) and quantitation (LOQ), solutions of concentration in the lower part of the linear range of the calibration plot were used. LOD and LOQ were calculated using the equations LOD = 3.3 �� ��/S and LOQ = 10 �� ��/S, where �� is the standard deviation of the peak areas of the drugs (n = 3), and S is the slope of the calibration plot in the lower part of linear range.