“
“Viruses in the Ebolavirus and Marburgvirus genera (family Filoviridae) have been associated with large outbreaks of hemorrhagic fever in human and nonhuman primates. The first documented cases occurred in primates over 45 years

ago, but the amount of virus genetic diversity detected within bat populations, which have recently been identified as potential reservoir hosts, suggests check details that the filoviruses are much older. Here, detailed Bayesian coalescent phylogenetic analyses are performed on 97 whole-genome sequences, 55 of which are newly reported, to comprehensively examine molecular evolutionary rates and estimate dates of common ancestry for viruses within the family Filoviridae. Molecular evolutionary rates for viruses belonging to different species range from 0.46 x 10(-4) nucleotide substitutions/site/year for Sudan ebolavirus to 8.21 x 10(-4) nucleotide substitutions/ site/year for Reston ebolavirus. Most recent common ancestry can be traced back only within the last 50 years for Reston ebolavirus and Zaire ebolavirus species and suggests that viruses within these species may have undergone recent genetic bottlenecks. Viruses within Marburg marburgvirus and Sudan ebolavirus species can be traced back further and share most recent common ancestors approximately 700 and 850 years before the present, respectively. Examination of the whole family suggests that members of the Filoviridae, PRT062607 purchase including

the recently described Lloviu virus, shared a

most recent common ancestor approximately 10,000 years ago. These data will be valuable for understanding the evolution of filoviruses in the context of natural history as new reservoir hosts are identified and, further, for determining mechanisms of emergence, pathogenicity, and the ongoing threat to public health.”
“The mitotic spindle faithfully separates the genetic material, and has been reverently observed for well over a century. Across eukaryotes, while the Evofosfamide concentration mechanisms for moving chromosomes seem quite conserved, mechanisms for assembling the spindle often seem distinct. Two major pathways for spindle assembly are known, one based on centrosomes and the other based on chromatin, and these pathways are usually considered to be fundamentally different. We review observations of spindle assembly in animals, fungi, and plants, and argue that microtubule assembly at a particular location, centrosomes, or chromatin, reflects contingent, cell-type specific factors, rather than reflecting a fundamental distinction in the process of spindle building. We hypothesize that the essential process for spindle assembly is the motor-driven organization of microtubules that accumulate in the form of dense bundles at or near the chromosomes.”
“Little is known concerning immunodominance within the CD4 T-cell response to viral infections and its persistence into long-term memory.