The hydrolytic potential of B. firmus and B. indicus genomes correlates with growth on selected carbohydrates The CAZy annotation results were compared

to the growth profile of B. firmus GB1 and B. indicus HU36 (Table 2). Overall the growth profiles of both strains on minimal medium supplemented with selected monosaccharides, disaccharides or cellulose correlated with the presence of related CAZymes in their genome (Additional Files 1 and 2). B. firmus GB1 buy AZD5363 was able to grow efficiently in minimal medium supplemented with glucose, fructose, arabinose, mannose, xylose, sucrose or trehalose, as expected by the presence of candidate specific GHs (Additional File 4). Weak growth was observed with galactose, lactose,

maltose and cellulose, while growth was not supported only by fucose (Table 2 and Additional File 4). B. indicus HU36 was able to grow efficiently in minimal medium supplemented with glucose, fructose, mannose, maltose, sucrose or trehalose, as expected by the presence of candidate specific GHs (Additional File 4). Weak growth was supported by galactose while growth was not observed in the presence of arabinose, fucose, xylose, lactose or cellulose as sole carbon sources in agreement with the absence of candidate specific GHs (Table Bafilomycin A1 nmr 2 and Additional File 4). Table 2 Growth and pigment formation in minimal and rich media   Bacillus firmus GB1 Bacillus

indicus HU36   Minimal medium a Rich medium b Minimal medium a Rich medium b   growth pigment growth pigment growth pigment growth pigment NO SUGAR – - + + – - + + Glucose + – + – + – + – Fructose + – + – + – + – Galactose +/- – + + +/- – + + Arabinose + – + – - – + + Mannose + – + – + – + – Fucose – - + + – - + + Xylose + – + – - – + + Lactose +/- – + +/- – - + + Maltose +/- – + +/- + – + – Sucrose + – + – + – + – Trehalose + – + – + – + – Cellulose +/- Sitaxentan – + +/- – - + + a M9 minimal medium; bLB rich medium. We never observed carotenoid formation in solid minimal medium supplemented with any of the carbohydrate analyzed (Table 2). When the same selected carbohydrates were used to supplement rich (LB) medium, growth was always allowed but carotenoid formation was inhibited by all sugars able to support efficient growth as sole carbon source (Table 2). Galactose that, as sole carbon source, weakly supported growth of both B. firmus and B. indicus did not affect carotenoid synthesis in either organisms (Table 2), while lactose, maltose and cellulose were also able to support a weak growth of B. firmus and showed a partial negative effect on carotenoid production (Table 2). Results of Table 2 are, therefore, suggestive of a catabolite repression-like control on carotenoid biosynthesis in both pigmented Bacilli.