The present study was performed to learn which function DNA damage, necro sis or apoptosis provoked by extreme culture conditions, would induce very good results. In reality, it’s been proven that low bodily settings can produce genotoxic effects in cultured mammalian cells, specifically with respect to osmolality, ionic strength and pH. Apoptosis is really a type angiogenesis inhibitors list of cell death occurring under physiological conditions or in response to external stimuli, such as for example DNA damaging agents, development factor deprivation or death receptor triggering. It’s characterized by biochemical functions including the activation of cysteine proteases named caspases, mitochondrial permeability transition, cell membrane exposure of phosphatidyl serine, and DNA cleavage ultimately causing the normal morphology of apoptotic cells, in which the nucleus seems fragmented and condensed. In most cell types, DNA cleavage does occur after an irreversible activation of endonucleases. A short cleavage of DNA in to 50?300 kbp pieces induces chromatin condensation, and in most cell types an oligonucleosomal fragmentation uses due to double stranded cleavage of DNA in the linker region of nucleosomes. During the process of apoptosis and at the stage of chromatin condensation, the original nucleus breaks into Cholangiocarcinoma a number of thick micronuclei, spread through the cytoplasm. These micronuclei usually seem surrounded by a membrane system, externally outlined by ribosomes. The functional role of those micronuclei continues to be not known, nonetheless it is normally accepted which they contain sequestrated lazy genetic material. Subsequently, in the in vitro micronucleus check, a possible difficulty is that, using Giemsa staining, the early steps of chromatin condensation due to apoptosis are not easily distinguishable from micronuclei induced by chemicals. We used T lymphocytes of murine origin that over expresses the anti apoptotic protein Bcl2, if apoptosis is responsible for the clastogenicity observed under extreme culture problems to handle the question. This cell line was once used to demonstrate interference of apoptosis in the micronucleus assay. Bcl2 was selected natural product library since it protects the cells against several additional apoptotic stimuli: UV, radiation, genotoxic agents, hormones. In the present work we compared results obtained in the CTLL 2 parental cell line with the one obtained in CTLL 2 cells transfected with the gene. Our results clearly show that treat ments with super osmotic or hypo osmotic medium, and treatments with low or high pH can induce chromosome aberrations in vitro.