Specifically, HPTLC is one of the ideal TLC techniques for analytical purposes because of its increased accuracy, reproducibility, and ability to document the results, compared with standard TLC. Because check this of this, HPTLC technologies are also the most appropriate TLC techniques for conformity with Good Manufacturing Practices (GMPs). Today, the comprehensive use of TLC in pharmaceutical analysis is demonstrated by the great number of articles published in this field. So the ultimate aim of the present study is to develop and validate the HPTLC method for the determination of mycophenolate mofetil in bulk drug and dosage form. The optimization of the method separation, validation parameters, and quantification of mycophenolate mofetil as bulk and as formulation are reported in the following sections.

MATERIALS AND METHODS Chemicals and reagents The authentic sample of mycophenolate mofetil was procured from Intas Pharmaceuticals Ltd., Ahmedabad. The pure drug obtained had 99.9% w/w assay value, and was used without further purification. All chemicals and reagents used were of analytical grade. Mycophenolate mofetil is available as commercial tablets under the brand name Mycofit 250 mg from Intas Pharmaceuticals Ltd., and Mycofit 250 mg was procured from the local pharmacy. Preparation of the standard stock solution Analyte (10, 20, 30, 40, and 50 mg) was accurately weighed and separately dissolved in methanol in 100 mL volumetric flasks to furnish solutions in the concentration range of 100�C500 ng ��L-1. These solutions were used for the working range.

Chromatographic conditions Chromatography was performed on 10 �� 10 cm aluminum plates precoated with 250 ��m layers of silica gel 60 F254 (E. Merck, Darmstadt, Germany). Before use, the plates were prewashed with methanol and activated at 110�� for five minutes. The samples were applied to the plates as bands that were 6 mm wide and 10 mm apart by means of a Camag Linomat V sample applicator (Camag, Muttenz, Switzerland) equipped with a 100 ��l syringe (Hamilton, Bonaduz, Switzerland). Linear ascending development was performed in a 10 �� 10 cm twin trough glass chamber (Camag), with toluene, acetone, and methanol in the ratio 6:2:2 (v/v/v) as the mobile phase and the chamber was presaturated with mobile phase vapor for 10 minutes. The development distance was 8.5 cm with a development time of approximately 60 minutes. Drug_discovery After chromatography, the plates were dried in a current of air by using air-blowing drier. Densitometric scanning was performed with a Camag TLC Scanner 3 at 254 nm for all measurements. The scanner was operated by Wincats software version 1.2.3. The source of radiation was a deuterium lamp emitting a continuous ultraviolet (UV) spectrum between 200 and 400 nm.