Several methods have been figure 1 developed for measuring the total antioxidant capacity of foods and beverages selleck [13, 14]. High performance liquid Inhibitors,Modulators,Libraries chromatography coupled with an electrochemical multichannel detector (HPLC-ED) has been utilized for determination Inhibitors,Modulators,Libraries of the content of bioactive phenols. Electrochemical analysis using liquid chromatography of the total content of antioxidative active phenolic acids and polyhydroxylated derivatives of flavan Inhibitors,Modulators,Libraries was the technique selected for TAC determination in the extracts of the berries of less common fruit species. Peaks showing detected antioxidant complex(es) were summarized and their areas were then integrated (derived) to obtain the relative values.
Obtained data (%) were interpreted as relative antioxidant capacity [15].
Because different antioxidant compounds may act in vivo via different mechanisms, no Inhibitors,Modulators,Libraries single method can fully evaluate the TAC of Inhibitors,Modulators,Libraries foods [11, 16]. The reference Inhibitors,Modulators,Libraries method used for the verification of the results obtained from the HPLC-ED analysis was the DPPH? test. Among the fruits such as apricots or peaches belonging to the Rosaceae, Hawthorn fruit (Crataegus pinnatifida Bunge), Inhibitors,Modulators,Libraries whose analytic profile has not been compiled yet, showed the highest antioxidant capacity by both methods (Figure 1). This observation is in good agreement with the higher phenolic content of Hawthorn fruit compared to other stone fruits rpdiuced by the Rosaceae described by Ercisli [17].
A relatively high level of antioxidative capacity was found in the extracts from Honeysuckle berries (Lonicera edulis, Turcz. ex. Freyn).
Such a high level of antioxidant capacity is in agreement with the literature and is likely due to the high content Inhibitors,Modulators,Libraries of phenolic AV-951 acids and flavonoids [16], which were discovered in the berries of different taxons of the genus Lonicera L. The sequence of berries in the graphs, drawn on the basis of the results from HPLC-ED and DPPH? tests, was different in the two samples (Figure 1). The different sequence in the graph of DPPH? test, in comparison with the sequence in the graph of HPLC-ED, was discovered in the sample of Shadbush var. Martin and the L-KL-21 Honeysuckle genotype (Figure 1).
The difference in the sequences, compiled on the basis of two different Cilengitide methods of evaluation of biological value, reflects high content of antioxidants with the absence selleckchem Tofacitinib of phenol rings in the extracts of Shadbush var.
Martin. In such cases, phenolic components, which have demonstrated strong antioxidant activities in different model systems [15, 18] do not represent the major antioxidant complex. However, even if different rankings of TAC were reported among these fruits, there is agreement all targets in the literature that Amelanchier fruit is one of the most effective of functional foods.Figure 1.HPLC-ED chromatograms of fruit samples.