Overexpression of PLA2G2A resulted in enhanced cancer cell growth, whereas

gene knockdown attenuated growth.

Conclusions: Group IIa secretory phospholipase A(2) appears significant in growth and proliferation of human esophageal adenocarcinoma cells. Secretory phospholipase A(2) inhibition should be studied further regarding potential chemopreventive and therapeutic properties in esophageal adenocarcinoma. ( J Thorac Cardiovasc Surg 2010; 139: 591-9)”
“P-type calcium channels play a key role in the synaptic transmission between mammalian central neurons since a major part of calcium entering pre-synaptic terminals is delivered via these channels. Using conventional whole-cell patch clamp techniques we have studied the effect of mu-opioids on P-type calcium channels in acutely isolated Purkinje neurons from rat cerebellum. The selective mu-opioid agonist DAMGO (10 nM) produced a small, but consistent facilitation of current LY3023414 mw through P-type calcium channels (10 +/- 1%, n = 27, p < 0.001). The effect of DAMGO was rapid (less than 10 s) and fully C646 supplier reversible. This effect was both concentration and voltage-dependent. The EC(50) for the effect of DAMGO was 1.3 +/- 0.4 nM and the saturating concentration was 100 nM. The endogenous selective agonist of mu-opioid receptors, endomorphin-1 demonstrated similar action. Intracellular perfusion of Purkinje neurons

with GTR-gamma S (0.5 mM) or GDP beta S (0.5 mM),

as well as strong depolarizing pre-pulses (+50 mV), did not eliminate facilitatory action of DAMGO on P-channels indicating that this effect is not mediated by G-proteins. Furthermore, the effect of DAMGO was preserved in the presence of a non-specific inhibitor of PKA and PKC (H7, 10 mu M) inside the cell. DAMGO-induced facilitation of P-current was almost completely abolished by the selective mu-opioid antagonist CTOP (100 nM). These observations indicate that mu-type opioid receptors modulate P-type calcium channels in Purkinje neurons via G-protein-independent mechanism. (C) 2010 Elsevier Ireland Ltd. All rights reserved.”
“Objective: Preventing air leaks after major lung resection for cancer is mandatory for successful fast-track surgical intervention. We reported our preliminary results with performance of pneumostasis by combining 4-Aminobutyrate aminotransferase polyglycolic acid mesh and fibrin glue; however, the advantages of this combination over the conventional method have not been clarified.

Methods: We controlled air leaks detected during an intraoperative water-seal test by using sutures and fibrin glue before April 2006 and by combining polyglycolic acid mesh and fibrin glue without sutures thereafter. We removed the chest tube the day after the air leaks stopped. For bias reduction in comparison with the 2 historical cohorts, we used the nearest available matching method with the estimated propensity score.