Microbiology 1997,143(Pt 11):3443–3450.PubMedCrossRef 27. Li J, Jensen SE: Nonribosomal biosynthesis of fusaricidins by Paenibacillus polymyxa PKB1 involves direct activation of a D-amino acid. Chem Biol 2008,15(2):118–127.PubMedCrossRef 28. Steller S, Sokoll A, Wilde

C, Bernhard F, Franke P, Vater J: Initiation Small molecule library solubility dmso of surfactin biosynthesis and the role of the SrfD-thioesterase protein. Biochemistry 2004,43(35):11331–11343.PubMedCrossRef Competing interests The authors declare that they have no competing interests. Authors’ contributions CDQ was responsible for designing the study, bioinformatic analysis, and writing the manuscript. CDQ and TZL performed the recombinant protein preparation and biochemical experiments. SLZ made substantial contributions to data analyses and interpretation. WPZ, RD, and OL helped to revise the manuscript. XCW was responsible for the integrity of the work as a whole. All authors read and approved the final manuscript.”
“Background The main cause of morbidity and mortality in cystic fibrosis (CF) is EVP4593 datasheet chronic lung disease caused by a vicious cycle of infection and inflammation Ruboxistaurin datasheet which leads to progressive deterioration of pulmonary function, respiratory failure, and death [1]. Pseudomonas aeruginosa is the main bacteria associated

with pulmonary disease in CF. In vivo and in vitro evidence suggests that P. aeruginosa produce biofilm within the airways of chronic CF pulmonary infection patients,[2–5] which is a protective barrier around the bacterial cells and limits exposure to oxidative radicals, antibiotics, and phagocytes [6]. Bacterial biofilms

play a relevant role in persistent infections, which are rarely eradicated with antimicrobial therapy [7]. Despite the evidence of P. aeruginosa grown in the airways of CF patients in biofilm form, the susceptibility profile of the bacterium is usually evaluated, in vitro, in the planktonic state. However, the planktonic susceptibility profile may not represent the actual susceptibility of the bacteria [7]. To overcome the potential shortfalls of traditional (planktonic) microbiological methods to evaluate susceptibility, biofilm models have been proposed to Silibinin access susceptibility of P. aeruginosa in vitro[8]. Macrolide antibiotics are being evaluated for the treatment of chronic lung inflammatory diseases, including diffuse panbronchiolitis, CF, chronic obstructive pulmonary disease, and asthma. Although macrolides have no antimicrobial activity against P. aeruginosa at therapeutic concentrations, there is great interest in the evaluation of treatments of CF patients with these antibiotics, at least as complementary therapy [9–11]. Anti-inflammatory activity of macrolides has been showed in many studies, including clinical trials [12–17].