methylohalidivorans DSM 14336T was also determined for this study using Generation-III microplates in an OmniLog phenotyping device (BIOLOG Inc., Hayward, CA, USA). The microplates were inoculated http://www.selleckchem.com/products/Enzastaurin.html at 28��C with a cell suspension at a cell density of 95-96% turbidity and dye IF-A. Further additives were vitamin, micronutrient and sea salt solutions. The exported measurement data were further analyzed with the opm package for R [28,29], using its functionality for statistically estimating parameters from the respiration curves such as the maximum height, and automatically translating these values into negative, ambiguous, and positive reactions. The strain was studied in two independent biological replicates, and reactions with a different behavior between the two repetitions were regarded as ambiguous and are not listed below.
The strain gave positive reactions for 1% NaCl, 4% NaCl, D-glucose, D-mannitol, D-glucose-6-phosphate, D-aspartic acid, L-alanine, L-arginine, L-glutamic acid, L-histidine, L-pyroglutamic acid, L-serine, D-galacturonic acid, glucuronamide, quinic acid, D-saccharic acid, D-lactic acid methyl ester, ��-keto-glutaric acid, L-malic acid, nalidixic acid, acetoacetic acid, propionic acid and acetic acid.
The strain was negative for sucrose, pH 6, pH 5, D-melibiose, D-salicin, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, N-acetyl-neuraminic acid, 8% NaCl, D-galactose, 3-O-methyl-D-glucose, D-fucose, L-fucose, inosine, 1% sodium lactate, fusidic acid, D-serine, D-sorbitol, D-arabitol, D-fructose-6-phosphate, D-serine, troleandomycin, rifamycin SV, minocycline, lincomycin, guanidine hydrochloride, niaproof 4, pectin, L-galactonic acid-��-lactone, mucic acid, vancomycin, tetrazolium violet, tetrazolium blue, p-hydroxy-phenylacetic acid, methyl pyruvate, citric acid, bromo-succinic acid, potassium tellurite, ��-hydroxy-butyric acid, ��-hydroxy-butyric acid, ��-keto-butyric acid, sodium formate, aztreonam, butyric acid and sodium bromate. Regarding the common subset of growth GSK-3 experiments and OmniLog experiments, the results were identical with few exceptions. Expectedly [30], on some substrates respiration was detected by phenotype microarray analysis even though these substrates did not sustain growth. Chemotaxonomy The major respiratory lipoquinone present is Q10 [1]. The polar lipids comprise phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid, two unidentified lipids and an aminolipid. Phosphatidylcholine is not present. The fatty acids comprise C10:0 3-OH, C14:1, C12:0 3-OH, C16:0, C16:0 2-OH, C18:1��9c, C18:1��7c, C18:0 and 11-methyl C18:1��7c. The C10:0 3-OH and C16:0 3-OH fatty acids are ester-linked, while the C12:0 3-OH fatty acid is amide-linked [2].