In infected C57BL/6J mice, Retnla increased between 18 h and 120 h (panel D). In mock treated mice of this strain, expression increased steadily at the early time check details points, with significant regulation at 18 h and 24 h.

This suggested a procedure-dependent regulation of Retnla resembling that observed in the DBA/2J mice. Irg1 mRNA expression increased in mock-treated DBA/2J mice between 6 and 18 h (panel E). This gene was up-regulated PLX3397 in vivo in DBA/2J infected mice at all time points, reaching a maximal 630-fold induction on day 2. In the C57BL/6J strain there was no increase in Irg1 due to mock treatment, and the infection-dependent increase was less pronounced, reaching a max. 150-fold induction at 120 h. Il6 mRNA increased in both strains beginning 6 h after infection or mock treatment, with stronger regulation being observed in the DBA/2J mice (panel G). In DBA/2J mice the mock treatment effect declined towards 18 h, and clear differences between infected and mock treated mice

became apparent at 24 h. In the C57BL/6J mice, an infection-dependent rise in Il6 mRNA was observed somewhat later (t = 48 h) (panel H). Il1b Selleckchem CFTRinh-172 mRNA increased in infected mice of both strains at 48 h and 120 h, and there was a tendency (p at 6 h = 0.09) toward a mock treatment effect between 6 and 18 h in the DBA/2J strain (panel I). Cxcl10 mRNA was up-regulated in DBA/2J mock-treated mice at 6 h (panel K), whereas it was not affected by mock treatment in the C57BL/6J mice (panel

L). In both mouse strains Cxcl10 mRNA was significantly elevated in the infected mice, beginning at 6 h in the DBA/2J and at 18 h in C57BL/6J. Stat1 expression was not affected by mock treatment in DBA/2J mice, but there was a slight trend (statistically not significant) for up-regulation in C57BL/6J mice. An infection-dependent up-regulation became apparent at 24 h and 48 h in DBA/2J and C57BL/6J mice, respectively. Similar to Stat1, Ifng Isotretinoin was up-regulated in both mouse strains beginning around 48 h, and there was no evidence for regulation due to the infection procedure. Ifnl2 was not detected (Ct ≥ 40) in about 40% of untreated and mock treated DBA/2J mice; fold change values therefore represent an underestimation (panel Q). A significant rise after infection became apparent at 48 h, reaching a mean Ct of 26.3. In C57BL/6J mice, it was not detected in about 80% of the untreated and mock treated samples, suggesting a lower baseline expression than in DBA/2J (panel R). A first significant infection-dependent regulation was observed at 18 h, where Ifnl2 was detected in all DBA/2J and four of five C57BL/6J samples. Ifnl2 was detected in all 24 h samples (Ct = approx. 33) and continued to rise through 120 h. There was no evidence for a mock treatment effect on Ifnl2 in either mouse strain. Mx1 mRNA expression (panels S and T) was not regulated in response to mock treatment in either strain.