Easton et al. (2007) were the first to add Gly to a Cr selleck kinase inhibitor containing solution and demonstrate that a combination of the two hyperhydrating agents has an additive effect, as the addition of Gly to Cr significantly increased TBW more than Cr alone. Although the combination of the aforementioned hyperhydrating agents results in an increase in TBW and a reduction in certain cardiovascular and thermoregulatory responses [19], the BM increase due to enhanced hydration Caspase inhibitor status could potentially reduce RE. The reduction of the energy cost of movement at a sub-maximal velocity by way of reducing BM to improve running performance is well known [20]. For instance,

it is noted that some marathon runners perform well despite dehydration of 4-8% BM [21]. Coyle [3] proposed that this may occur because fluid loss (i.e., reduced find protocol body mass) lowers the oxygen cost of movement. On the other hand, the acute influences of hyperhydration on RE has not been investigation to date. Hence, the aim of the present study was to investigate the effects of hyperhydration induced by a combined Cr and Gly supplementation on thermoregulatory and cardiovascular responses and RE during 30 min of running at a running speed corresponding to 60% in cool

(10°C with a relative humidity of 70%) and hot conditions (35°C with a relative humidity of 70%) in well trained male athletes. In cool ambient conditions were intended to minimize heat stress during exercise this enabling a focus on the effects of the altered BM induced by hyperhydration on RE at 60% . However, effects of hyperhydration on thermoregulatory and cardiovascular responses are also expected

during exercise in hot and humid conditions; conditions typical of major sporting events (e.g., Olympic Summer Games). As such, it was hypothesized that diglyceride an increase in BM and TBW induced by hydrating agents such as Gly or Cr would improve thermoregulatory and cardiovascular responses in line with previous findings but potentially negatively influence RE during running in the heat. Methods Subjects Fifteen trained male runners gave their written informed consent to take part in the present study which was approved by the University of Glasgow Ethics Committee and was performed according to the code of ethics of the World Medical Association (Declaration of Helsinki). One subject withdrew from the study before the final trial because of gastrointestinal distress during supplementation. Subjects were questioned as to their supplementation and training practices in order to ascertain that they had not supplemented with Cr for at least 8 weeks prior to commencing the study. Subjects were in good health at the time of testing, ran on a daily basis and participated regularly in competitive races. Athletes were also requested to maintain their typical weekly training regime during the course of the study.