Detection of ?NO2 generated in cytosols isolated from the pancreas of the rat intoxicated with l-arg was successful, however, ?NO has been found not to interact with two anomers of methyl 3-amino-2,3-dideoxyhexopyranoside, i.e., with ��-d-arabino (AaraNH2) [9] and ��-d-arabino (BaraNH2) configurations [10�C12]. While carrying out the measurements of ?NO2 uptake by the two complex ions cis-[Cr(C2O4)(AaraNH2)(OH2)2]+ or cis-[Cr(C2O4)(BaraNH2)(OH2)2]+ it was noted that for all studied samples the approximated curve decayed biexponentially. It should be stressed that the studied reaction proceeded in two steps. The observable rate constants, for the first (k1obs) and second steps (k2obs), were obtained by fitting the rate data to the same consecutive reaction model.

Global value analysis of the observable rate constants for both steps was based on the model of consecutive reactions of type: A ��B ����C. In the first step, an intermediate compound B was formed and subsequently converted to a final product C, characteristic for the second step. The same reaction pathway in the biological sample was confirmed by the spectral and kinetic analysis for both complexes. Nitrogen dioxide levels in the cytosolic fraction of pancreatic acinar cells were determined on the basis of a standard curve created according to the method of Jacewicz et al. [12].2.?Results and Disscusion2.1.

Kinetics of ?NO2 Uptake Generated from Pancreatic Cytosol FractionsThe presented study is a continuation of our kinetic studies on the mechanisms of gas uptake reactions by Cr(III) co-ordination compounds with bioactive ligands [8].

The selection of chromium(III) as the center of co-ordination permits the creation of inert complexes that undergo relatively slow transformations at ambient temperature, thus facilitating investigations on the kinetics and mechanism of the studied processes. The synthesis of anomeric methyl 3-amino-2,3-dideoxy-��-d-arabino-hexopyranoside co-coordinated to Cr(III) turned out to be successful Carfilzomib for the detection of ?NO2 generated in the cytosol isolated from the pancreas of rats intoxicated with L-arg. Importantly, ?NO has been found not to interact with this ligand.

While carrying out measurements of ?NO2 uptake by cis-[Cr(C2O4)(AaraNH2)(OH2)2]+, Anacetrapib it was noted that for all studied cytosol samples the approximated curve decayed bi-exponentially. It should be stressed that the studied reaction proceeded in two steps. The observable rate constants, k1obs for step 1 and k2obs for step 2, were obtained by fitting the rate data to the consecutive reaction model.