Although the breakfast might mask the potential benefit of the supplementation during the recovery period, it more closely reflects the real-life behavior of athletes as they rarely participate in matches in a fasted state. The amount of BCAA consumed in this study, 7 g in a 70-kg subject, was similar to the 6.5-15.8 g dosages ingested before exercise in the literature [60–62]. The amount of arginine consumed in this study, 7 g

in a 70-kg subject, has been shown to result in a significant improvement of flow-mediated vasodilatation [63]. In addition, it has been suggested that post-exercise supplementation of 0.3-0.5 g total protein/kg/hr could produce higher insulinemic responses [38]. Since whey protein hydrolyate MDV3100 research buy containes approximately 13.4% amino acids as BCAA and arginine [17], we selected 0.1 g amino acids/kg/hr

in this study. A limitation of this study is that muscle biopsy was not performed because it would interfere with the performance in the subsequent exercise. Future studies with modified protocols may allow the biopsy procedure and further clarify the effect of BCAA and arginine on post-exercise glycogen recovery. Another limitation of this study is that inflammatory response was not measured. Strenuous exercise such as the simulated match in this study could result in significant inflammatory response and muscle damage. However, there www.selleck.co.jp/products/s-gsk1349572.html was no significant difference in plasma concentrations of creatine kinase and lactate dehydrogenase at the baseline selleck screening library among the 3 trials (data not shown). It is reasonable to assume that the 2-week period between each trial is sufficient for the subjects to recover completely. The other mechanisms that may affect the performance in multiple wrestling matches, such as neuromuscular and/or psychological fatigue, were not investigated in this study and could be involved in future studies. Conclusions In learn more conclusion, this study suggested that supplementation of carbohydrate with or without BCAA and arginine during the post-match period

did not provide additional effect on the performance in the following simulated match in well-trained male wrestlers when a carbohydrate-rich breakfast was eaten. It is possible that factors other than muscle glycogen content contribute to the performance in multiple bouts of high-intensity intermittent exercise. It is also possible that experienced wrestlers have the ability to recovery quickly from previous matches with or without supplementation. Furthermore, BCAA and arginine did not provide additional insulinemic effect when given after high-intensity intermittent exercise. Acknowledgements and funding We gratefully acknowledge the technical assistance of Mei-Hui Tseng and I-Fan Chen and the enthusiastic support of the subjects who volunteered to participate in this study.