Moreover, learn more it has been proved that this technology can be useful to decompose some toxins that are not affected by thermal processing. In this piece of work, the effect of UV irradiation on all of these

contaminants and components of liquid food has been reviewed. (C) 2011 Elsevier Ltd. All rights reserved.”
“A novel and simple method for the preparation of chemically bonded poly(vinyl alcohol) (PVA) coating to silica capillary inner wall was developed, and the PVA-coated capillary columns were employed for capillary electrophoresis (CE). The coating procedure included pretreatment of the capillary inner wall, silanization, aldehyde group functionalization and PVA immobilization. Electroosmotic flow of the coated capillary was almost suppressed over a wide pH range (pH 3-10). High-efficiency separations of cationic proteins (including cytochrome c, lysozyme, a-chymotrypsinogen A) at pH 3.0-5.0 and of anionic proteins (including myoglobin and trypsin inhibitor) at pH 10.0 were achieved with the PVA-coated capillary. Moreover, a “”dual-opposite-injection”" approach was adopted for simultaneous separations of both cationic and anionic proteins at neutral pH with the prepared column. In this CE mode, positively charged proteins migrated from one end of the column to the detector this website while negatively charged proteins from

the other end to the detection window. Good run-to-run repeatability was obtained in all of the protein CE separations performed in this work. The PVA-coated

column can also afford long-term stable uses for protein separations, as demonstrated in 100 repeated uses using a single capillary with the relative standard BI 10773 datasheet deviation values of the retention times less than 0.9%. Moreover, good column-to-column reproducibility was demonstrated by protein CE separation with 10 different columns. The results indicate that the present method for PVA-coated capillary preparation is promising for protein CE applications. (C) 2010 Elsevier B.V. All rights reserved.”
“. miR-122 is a liver-specific microRNA, which also circulates in the blood. The levels of miR-122 in serum and plasma correlate with hepatic necroinflammation in patients with hepatitis B virus (HBV) infection. Here, we investigated whether miR-122 levels correlate with surrogate markers for viral replication and translation. Furthermore, we examined whether miR-122 levels differ in the different groups of HBV-infected patients and whether miR-122 levels may be useful to identify patients with higher or lower risk for liver disease progression. Therefore, RNA was extracted from sera of therapy-naive patients with HBV infection (n = 89) and from healthy volunteers (n = 19). The concentration of miR-122 was assessed by quantitative real-time reverse-transcription PCR. HBs antigen and HBV DNA levels were quantified as surrogate parameters for HBV replication and translation.