76; 95% CI, 1.21-2.56). rs4796793 G(CG+GG) allele was significantly associated with HBeAg seroconversion and inversely associated with high viral load (≥1 × 104 copies/mL) (AOR, 0.69; 95% CI, 0.56-0.86); rs2293152 CG genotype was significantly associated with cirrhosis (AOR, 2.41; 95% CI, 1.13-5.13) while its G(CG+GG) allele and GG genotype were significantly associated with high viral load (G allele: AOR, 2.28; 95% CI, 1.19-4.37; GG genotype: AOR, 2.73; 95% CI, 1.32-5.65,

respectively) in females; rs1053004 TC genotype was significantly associated with HCC-free chronic HBV infection (AOR, 1.25; 95% CI, 1.01-1.56), whereas its variant genotypes were inversely

PD0325901 supplier associated with high viral load (Supporting Table 2). We successfully amplified and sequenced the EnhII/BCP/PC region from 252 (79.75%) ASCs, 172 (54.43%) CHB patients, 224 (62.57%) cirrhosis patients, and 512 (50.15%) HCC patients as well as the preS region from 130 (41.14%) ASCs, 164 (51.90%) CHB patients, 194 (54.19%) cirrhosis patients, and 460 (45.05%) HCC patients (GenBank No. JX556943-JX559050). The “hotspots” in the EnhII/BCP/PC PARP inhibitor region and the preS region of HBV genotype C and their associations with HCC are listed in Supporting Tables 3 and 4, respectively. Of those, C1653T, T1674C/G, G1719T, A1727G, T1753C, A1762T/G1764A, A1846T, G1896A, C2875A, A1C/T, C7A, C10A, A31C/T, T49A, A52C/T, C76A, G105C/T, C109A/T, A135C, G147C, preS deletion, and preS2 start codon mutation were significantly associated with an increased risk of HCC, whereas A1652G, C1673T, A1726C, A1727T, C1730G, and C1799G were significantly associated with a reduced risk of HCC, compared with the HBV-infected subjects without HCC (after the

Bonferroni correction for multiple comparison). However, preS1 deletion, preS2 deletion, and T53C (F141L), the mutations reported to be related to HCC,4, 5, 7, 12 were not significantly associated with HCC. Of those HCC-related HBV mutations, A1762T/G1764A, G1719T, preS deletion, C10A, T49A, A135C, A1C/T, A31C/T, A52C/T, and C109A/T were more frequent in males than in females in HBV-infected patients without Abiraterone purchase HCC (Supporting Table 5). Correlation analyses indicated that the HBV mutations in the preS2 region including C10A, C31C/T, T49A, A52C/T, C109A/T, and A135C correlated with each other (phi > 0.800). Supporting Table 6 shows the correlations between the selected HCC-related mutations. In addition, G1896A, T1674C/G, C2875A, and C76A were significantly associated with HBeAg seroconversion; A1762T/G1764A was significantly associated with high viral load (AOR, 1.64; 95% CI, 1.18-2.28) and abnormal ALT (AOR, 1.94; 95% CI, 1.37-2.74).