1% and 56% of cells expressing the ecto-F1F0-ATPase β subunit. We prepared a McAb against the ecto-F1F0-ATPase β subunit, which significantly inhibited proliferation and induced apoptosis in cell lines derived from AML in vitro. These findings indicate that expression of the ecto-F1F0-ATPase β subunit is a

cancer-associated antigen in hematological malignancies. The ecto-F1F0-ATPase β subunit provides a Tubastatin A cell line potential target for immunotherapy in AML and other hematological malignancies. Acknowledgements We thank Professor Zhi-Hua Yang (Cancer Institute/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China) for her kindly instruction. This work was supported by grants from the National Key Basic Research Program No. 2010CB933902, National Selleckchem H 89 Natural Science Foundation for youth PLX4032 cell line No. 81100371, Natural Science Foundation of Jiangsu Province No. BK2011308, Universities Natural Science Foundation of Jiangsu Province No. 11KJB320014 and Talent’s subsidy project in science and education of department of public health of Suzhou City No. SWKQ1020. Medical innovation team and leading talent of Jiangsu Province. No. LJ201126. Major scientific

and technological special project for “significant new drugs creation” No. 2012ZX09103301-040. References 1. Valenti D, Tullo A, Caratozzolo MF, Merafina RS, Scartezzini P, Marra E, Vacca triclocarban RA: Impairment of F1F0-ATPase, adenine nucleotide translocator and adenylate kinase causes mitochondrial energy deficit in human skin fibroblasts with chromosome 21 trisomy.

Biochem J 2010, 431:299–310.PubMedCrossRef 2. Percy JM, Pryde JG, Apps DK: Isolation of ATPase I, the proton pump of chromaffin-granule membranes. Biochem J 1985, 231:557–564.PubMed 3. Zhang X, Gao F, Yu LL, Peng Y, Liu HH, Liu JY, Yin M, Ni J: Dual functions of a monoclonal antibody against cell surface F1F0 ATP synthase on both HUVEC and tumor cells. Acta Pharmacol Sin 2008, 29:942–950.PubMedCrossRef 4. Chi SL, Wahl ML, Mowery YM, Shan S, Mukhopadhyay S, Hilderbrand SC, Kenan DJ, Lipes BD, Johnson CE, Marusich MF, et al.: Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase. Cancer Res 2007, 67:4716–4724.PubMedCrossRef 5. Moser TL, Stack MS, Asplin I, Enghild JJ, Hojrup P, Everitt L, Hubchak S, Schnaper HW, Pizzo SV: Angiostatin binds ATP synthase on the surface of human endothelial cells. Proc Natl Acad Sci U S A 1999, 96:2811–2816.PubMedCrossRef 6. Radojkovic C, Genoux A, Pons V, Combes G, de Jonge H, Champagne E, Rolland C, Perret B, Collet X, Terce F, Martinez LO: Stimulation of cell surface F1-ATPase activity by apolipoprotein A-I inhibits endothelial cell apoptosis and promotes proliferation. Arterioscler Thromb Vasc Biol 2009, 29:1125–1130.PubMedCrossRef 7. Zick M, Rabl R, Reichert AS: Cristae formation-linking ultrastructure and function of mitochondria.